Journal: PLoS ONE

Article Title: Searching for Cellular Partners of Hantaviral Nonstructural Protein NSs: Y2H Screening of Mouse cDNA Library and Analysis of Cellular Interactome

doi: 10.1371/journal.pone.0034307

Figure Lengend Snippet: Intersection of closest shared TULV and PUUV NSs -linked nodes. Primary nodes are in red, secondary nodes in pink. Secondary nodes that do not directly connect to two different primary nodes are in white. Metanodes are represented by pink diamonds.

Article Snippet: Samples were incubated 1 h at 37°C with rabbit polyclonal antibodies against TULV NSs-GST fusion protein and mouse monoclonal anti-ACBD3 antibody (Abnova, Taipei, Taiwan).

Techniques:

Journal: PLoS ONE

Article Title: Searching for Cellular Partners of Hantaviral Nonstructural Protein NSs: Y2H Screening of Mouse cDNA Library and Analysis of Cellular Interactome

doi: 10.1371/journal.pone.0034307

Figure Lengend Snippet: Primary cells were infected with TULV strain Lodz for 9 days and fixed on coverslips. Cells were stained for the viral NSs protein, which was seen as bright spots around perinuclear area (A), and ACBD3 protein (B). FRET assay: D pre , donor intensity before bleaching, D post , donor intensity after bleaching, A pre , acceptor intensity before bleaching, A post , acceptor intensity after bleaching, FRET eff , calculated efficiency of FRET.

Article Snippet: Samples were incubated 1 h at 37°C with rabbit polyclonal antibodies against TULV NSs-GST fusion protein and mouse monoclonal anti-ACBD3 antibody (Abnova, Taipei, Taiwan).

Techniques: Infection, Staining

Journal: American Journal of Human Genetics

Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

doi: 10.1016/j.ajhg.2010.04.017

Figure Lengend Snippet: The Kir3.4-Gly387Arg Mutation Is Associated with Congenital Long QT Syndrome

Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na + /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

Techniques: Mutagenesis

Journal: American Journal of Human Genetics

Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

doi: 10.1016/j.ajhg.2010.04.017

Figure Lengend Snippet: Clinical Characteristics of the Pedigree Members with Kir3.4-Gly387Arg or Sudden Death

Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na + /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

Techniques:

Journal: American Journal of Human Genetics

Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

doi: 10.1016/j.ajhg.2010.04.017

Figure Lengend Snippet: Electrophysiological Analyses of Kir3.4-Gly387Arg

Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na + /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

Techniques:

Journal: American Journal of Human Genetics

Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

doi: 10.1016/j.ajhg.2010.04.017

Figure Lengend Snippet: Cell Surface Expression of Kir3.4-Gly387Arg and Kir3.1

Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na + /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

Techniques: Expressing

Journal: American Journal of Human Genetics

Article Title: Identification of a Kir3.4 Mutation in Congenital Long QT Syndrome

doi: 10.1016/j.ajhg.2010.04.017

Figure Lengend Snippet: Human Atrial and Ventricular Expression of Kir3.1 and Kir3.4

Article Snippet: Proteins were transferred onto Hybond-P polyvinylidene difluoride transfer membranes (Amersham Biosciences, 0.45 μm), and membranes were blocked in 5% nonfat milk in TBST (0.1% Tween 20 in Tris-buffered saline solution) for 2 hr at room temperature and then incubated with primary antibody against Kir3.1 (1:1,000; Alomone Labs), Kir3.4 (1:1,000; Alomone Labs), calnexin (1:2,000; Nordic Biosite), or Na + /K+-ATPase α1 (1:500; Santa Cruz Biotechnology) overnight.

Techniques: Expressing